Bioneovan Co.,Ltd

Rotavirus rapid diagnostic test kit

Rapid diagnostic test kit

The kit uses a sandwich solid phase immunochromatographic assay to detect group A rotavirus antigen in human stool specimen. Nitrocellulose-based membrane pre-coated with Goat polyclonal anti-rotavirus antibodies and the gold labeled specially-selected mouse monoclonal anti-rotavirus antibodies are used as detector materials, respectively. Add an aliquot of diluted stool sample to the sample well of the test cassette. If the sample contains rotavirus antigens, a antigen-antibody-gold complexes will form. These complexes move on the nitrocellulose membrane by capillary action toward the test line region on which rotavirus specific antibodies are immobilized. As the complexes reach the test line, they will bind to the antibody on the membrane in the form of a pink line. A second red control line will always appear in the result window to indicate that the test has been correctly performed and the test device functions properly. If rotavirus antigen is not present or lower than the detection limit of the test, only the control line will be visible. If the control line dose not developed, the test is invalid. The test results are intended to aid in the diagnosis of rotavirus infection and to monitor the effectiveness of therapeutic treatment. The test is fast and easy to operate.

1. Bring Membrane Plate and samples to room temperature (20-30°C) before use (approximately 30 minutes).

2. Remove the test cassette/strip from the sealed foil pouch and place the plate on flat desk.

3. Bring all materials and specimens to room temperature (8~30°C).

4.1 Group A Rotavirus test strip

1) Add 500μl sample diluents into the test tube.

2) Add proper specimens (S:5 mm in diameter, L: 50μl) into the tube to make the concentration of the solution up to 5%~10% and mix thoroughly.

3) Deliver 100μl (2~3drops ) of diluted stool sample to the sample pad of the test strip.

4.2 Group A Rotavirus test cassette

1) To take a portion of feces (about 100mg), insert the sterile swab into a stool sample that presents the most secretion under visual inspection.

2) Open the sample collection tube and then insert the swab into the sample collection tube containing assay diluents.

3) Swirl the swab at least 10 times until the samples has been dissolved into the assay diluents and discard the swab while squeezing the swab against the wall of tube, replace the cap.

4) Deliver 100μl (2~3drops ) of diluted stool sample to the sample well of the test card.

5. Read the result in 5~10 minutes. A strong positive sample may show result earlier.

Note: Results after 15 minutes may not be accurate.

Bioneovan was built in 1988 in Beijing, China and was the earliest domestic high-tech enterprise which was specialized in the field of ELISA TESTS & RAPID TESTS as both supplier and manufacturer with GMP, ISO9001:2008, and ISO13485 Certificates.

Our products mainly go through the following tests: 
1.Elisa : Hbsag elisa kit, Hbsab, Hbeag, Hbeab, Hbcab, Anti-HCV, HAV igm, HEV Igm, HEV Igg, Anti-HIV1&2, Anti-hiv Ab/Ag elisa test kit and so on.
2. Rapid test : Hbsag cassette hbsag strip, hcv rapid test cassette, hcv rapid test strip, hev igm cassette, hev igg cassette, hav igm cassette, h-pylori ag cassette and other 40 kinds of rapid test cassette or strip.